| 1. | Expression of crylac in transgenic tobacco plants was assayed with elisa . the results showed that pinll signal peptide sequence enhanced the expression of crylac protein in transgenic tobacco
荧光显微观察到gfp在植物细胞间隙高效表达， westernblot结果显示crylac蛋白也在植物细胞间隙表达。 |
| 2. | Gfp gene was also fused behind the signal peptide sequence to construct plasmid p3301ubisiggfp and transformed to tobacco . the results of fluorescent detection showed gfp localization in the apoplast
Elisa结果显示，马铃薯蛋白酶抑制剂基因的信号肽序列使crylac基因在转基因烟草中的表达量显著提高。 |
| 3. | We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene , 2 . 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3 . 0
我们直接将含有4个内含子和自身信号肽的2 . 4kb全长人生长激素基因直接克隆至真核表达载体pcdna3 . 0 ，然后利用脂质体转染家蚕bmn细胞，瞬时表达hgh 。 |
| 4. | To allow secretion of the crylac protein into the intercellular space , potato proteinase inhibitor ii ( pinll ) signal peptide sequence was n - terminally fused to the crylac coding region to construct plasmid p3301ubisigac
运用pcr技术克隆了马铃薯蛋白酶抑制剂基因的信号肽序列，并将其分别连到crylac 、 gfp基因的5 ’端，构建植物转化载体p3301ubisigac和p3301ubisiggfp 。 |
| 5. | In order to explore transgenic plants for production of recombinant scfv specific for presl ( 20 - 47 ) , nicotiana tabacum was transformed with a gene encoding anti - presl of hepatitis b surface antigen scfv and bearing an / / - terminal endoplasmic reticulum protein signal peptide sequence
在用烟草作为生物反应器时，分别将该单链抗体靶向细胞质和内质网。经westernblot分析，靶向细胞质中表达时，可溶单链抗体最高占总的可溶蛋白的0 . 06 。 |
| 6. | ( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed
( 3 )翻译后水平通过pcr扩增的方式在t - pa基因5端添加了真核分泌信号肽序列和植物翻译起始共有序列aaca ，在3端添加了内质网定位序列kdel ，构建了植物表达载体pbemt 。 |
| 7. | The new synthesized protein was led to endoplastic reticulum cavity by eukaryotic secretory signal peptide sequence and then anchored to innerwall of endoplastic reticulum by kdel sequence , which interdicted the process of protein entering golgi body and cytoplasm , and then avoided heterogeneous glycosylation modification of foreign protein and prolonged the disappearance of half life of protein in organism . 2
真核分泌信号肽序列可以引导新合成的蛋白质进入内质网腔， kdel序列将进入内质网腔的蛋白质锚定在内质网内壁上，从而阻断了蛋白质进入高尔基体和细胞质的过程，进而避免了外源蛋白质的异源糖基化修饰，延长了蛋白质在生物体内的半衰期。 |
| 8. | The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene , this intron contains donor sequence - gtatgc , lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene . this gene encodes a peptide of 467amino acid residues with molecular weight of 51 . 37kda , containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide
核苷酸序列分析表明， pcr扩增产物中包含有完整的phya基因，该基因全长1506bp ，其中包含一段长102bp的内含子，该内含子具有真菌植酸酶基因内含子的特征保守序列： donor序列? gtatgc ， lariat序列? gctgac及acceptor序列? cag 。该基因编码467个氨基酸，理论分子量为51 . 37kda ，其上有13个潜在的n -糖基化位点， n端19个氨基酸为信号肽序列，植酸酶活性位点序列( crvtfaqvlsrhgaryptdskgk )位于氨基酸序列的+ 71 + 93 。 |
| 9. | In comparison with genbank data , the homologies of the nucleotide sequence and amino acid sequence were as following : hc was 98 . 4 % and 100 % ; ha was 97 . 2 % and 99 . 3 % respectively . 4 . two recombinant prokaryotic expression vector were constructed which has complete open reading occlusing initation codon , leader signal peptide sequence and termination codon
序列分析表明，所克隆获得的基因与genbank中已经登录的核苷酸和氨基酸的同源性分别为： hc98 . 4和100 ， ha97 . 2和99 . 3 ，证明本试验虫株与国外报道的同源性很高。 |
| 10. | Therefore , blys , its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity , neoplasia , or immunodeficiency syndromes . in this study , epo signal peptide sequence and hsblys gene were linked by soe method . the fusion product was cloned into eukaryotic plasmids . pcdna3 , pcdna3 . 1 , pefneo , respectively . meanwhile , the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i . thus the recombinant plasmid can be used as secreting plasmid expressing other gene
本实验通过3 ’端互补，进行引物延伸合成epo信号肽序列：信号肽和hsblys基因采用重叠延伸拼接法形成融合基因；融合基因分别插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核载体：引物延伸合成信号肽时，利用亮氨酸同义密码，将信号肽基因的倒数第二个密码突变，在重组载体上的信号肽序列之后，形成bln酶切位点，使三种载体成为分泌表达载体。 |
